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Part:BBa_K940002

Designed by: Moyin Odugbemi   Group: iGEM12_Westminster   (2012-09-25)

ALDH1A3 promoter in a pSB1C3 backbone

ALHDH1A3 is an isoform of aldehyde dehydrogenase; which is one of the enzymes involved in alcohol metabolism. Our construct contains the promoter for ALDH1A3 restricted with EcoR1 and PstI and then placed in the pSB1C3 backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 287
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 215
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 418
    Illegal NgoMIV site found at 674
    Illegal NgoMIV site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]


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